DNA-binding induces a major structural transition in a type I methyltransferase.
نویسندگان
چکیده
منابع مشابه
DNA binding and subunit interactions in the type I methyltransferase M.EcoR124I.
The type I DNA methyltransferase M.EcoR124I consists of two methylation subunits (HsdM) and one DNA recognition subunit (HsdS). When expressed independently, HsdS is insoluble, but this subunit can be obtained in soluble form as a GST fusion protein. We show that the HsdS subunit, even as a fusion protein, is unable to form a discrete complex with its DNA recognition sequence. When HsdM is adde...
متن کاملHigh resolution footprinting of a type I methyltransferase reveals a large structural distortion within the DNA recognition site.
The type I DNA methyltransferase M.EcoR124I is a multi-subunit enzyme that binds to the sequence GAAN6RTCG, transferring a methyl group from S-adenosyl methionine to a specific adenine on each DNA strand. We have investigated the protein-DNA interactions in the complex by DNase I and hydroxyl radical footprinting. The DNase I footprint is unusually large: the protein protects the DNA on both st...
متن کاملThe structure of M.EcoKI Type I DNA methyltransferase with a DNA mimic antirestriction protein
Type-I DNA restriction-modification (R/M) systems are important agents in limiting the transmission of mobile genetic elements responsible for spreading bacterial resistance to antibiotics. EcoKI, a Type I R/M enzyme from Escherichia coli, acts by methylation- and sequence-specific recognition, leading to either methylation of DNA or translocation and cutting at a random site, often hundreds of...
متن کاملAn Amazing Distortion in DNA Induced by a Methyltransferase I
Much of my work in biology has been driven by my early training in chemistry. When studying a new chemical compound, the first and most important thing is to determine its detailed molecular structure. For a molecular biologist that usually means determining some DNA sequence, since an accurate knowledge of sequence will then allow the proper design of experiments to examine function. I was fir...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1994
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1994.tb06915.x